FastQCFastQC Report
Sun 14 Apr 2019
SRR6576658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329315
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10420.3164143752941712No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6280.19069887493736998No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5550.16853164902904513No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5270.16002915142037258No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5090.15456326010051166No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4870.1478827262651261No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.13027040979001867No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4080.12389353658351426No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4060.12328621532575194No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3990.1211605909235838No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3930.11933862715029683No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3860.1172130027481287No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3830.11630202086148521No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3770.11448005708819824No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3760.11417639645931706No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3660.11113979017050545No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3530.10719220199505033No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT3500.10628122010840685No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA3480.10567389885064452No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3460.1050665775928822No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC3400.10324461381959524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7100.052.318511
CTACGCT500.001700439637.5992134
GGGTACC3950.036.8853037
GTACATG19200.036.734711
CAACGCA10400.035.701175
TACATGG19650.035.654172
ATCAACG10300.035.135183
ACATGGG19800.035.1305773
TGGGTAC4150.035.1076976
CATGGGT5900.035.050114
TCAACGC10350.034.965454
AACGCAG10550.034.748096
TACTCTA550.002714486634.18115
TAGGATC550.002714486634.18114
GGTACCT4200.033.5707248
ATGGGTA4500.033.421525
GTCTTAG902.5806725E-531.3469541
CATGGGG10200.031.3326764
CAGTCCG600.004157139531.3326769
ACGACTG300.00416013231.33267688-89
Produced by FastQC (version 0.11.2)