FastQCFastQC Report
Sun 14 Apr 2019
SRR6576657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374361
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12650.3379091304916912No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6980.18645104591557346No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6600.17630041590870843No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6420.17149222274756185No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5840.15599915589497837No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5480.14638276957268517No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5140.13730062693496384No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4600.12287604745152407No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4580.12234180376695222No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4500.12020482902866485No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4470.11940346350180707No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4460.11913634165952117No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4430.11833497613266339No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4350.11619800139437601No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC4050.10818434612579834No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG3950.10551312770293914No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3920.10471176217608139No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.10310903112236586No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3800.10150630006865033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC555.28488E-542.715433
ATGGGTA4400.036.3081175
GTATCAA10450.035.085681
TACATGG23250.033.156792
GTACATG23250.032.9546131
ACATGGG23000.032.8908843
GGTACCT4300.032.7816128
CATGGGG9050.031.6707784
GGGTACC4650.031.3246527
CATGGGT6000.031.324654
CTCGATA600.004163074331.3246594
GTACAAC650.00614522528.9266531
GTATAAC650.00614522528.9266531
ATGGGAG3100.028.7984685
AACGCAG12650.028.6007696
ATCAACG12650.028.2293323
GTATTAA1503.6794518E-828.2034871
TCAACGC12900.027.6822514
CAACGCA12950.027.5753695
TGGGTAC4950.027.5277256
Produced by FastQC (version 0.11.2)