FastQCFastQC Report
Sun 14 Apr 2019
SRR6576656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420124
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12860.30610010377888436No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7800.18565947196541974No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6770.16114290066742198No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6250.148765602536394No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6040.1437670782911712No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5610.13353200483666727No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5170.12305890641810513No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4990.11877445706505699No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4960.1180603821728823No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4740.11282383296360123No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4710.11210975807142652No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4670.1111576582151936No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4640.11044358332301893No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4560.10853938361055307No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4460.10615913396997077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8350.044.4647561
TACATGG21250.038.0403752
ACATGGG21150.037.5536043
GTACATG21700.037.2515221
CATGGGT5950.034.7544984
ATCAACG11000.034.1800423
TCAACGC11000.033.7527924
AACGCAG11200.033.5696876
ATATAGT851.7527665E-533.174756
ATTACAC1309.16225E-932.5367743
CATGGGG9350.032.1694534
CAACGCA11700.031.7333955
CTAGTAC1052.0620791E-631.3317073
GTACGTA600.004159061231.3317056
GTATTAC953.748E-529.6826691
TACGTAG804.777232E-429.3734767
ATGGGTA4750.028.6932475
ACGCAGA13600.027.6456247
GGTACCT4200.026.855758
GTCCTAA1406.3550397E-726.855751
Produced by FastQC (version 0.11.2)