FastQCFastQC Report
Sun 14 Apr 2019
SRR6576653_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576653_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323494
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6140.18980259293835433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.14436125554106105No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3380.10448416353935468No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3350.10355678930675685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG405.675234E-446.992274
GTATCAA9300.042.036811
GAATCCG450.001010612841.7967539
TACATGG18950.036.2051282
GTACATG18950.036.0407181
ACATGGG19150.034.600053
ATCAACG11550.033.7693373
AACGCAG11700.033.33646
CAACGCA12000.033.286195
TCAACGC12000.032.5029874
TCTATAC600.004159950731.328183
GTAGGAC953.7482387E-529.679333
CATGGGG7350.029.410134
TTAGTTC804.7779756E-429.370173
CATGGGT4550.028.9183224
CATGGGA9250.028.4493774
TATCAAC13900.028.0601332
TAATAGA856.8119745E-427.6425114
ACGCAGA14100.027.328847
GTATAGT700.00873441726.9151361
Produced by FastQC (version 0.11.2)