FastQCFastQC Report
Sun 14 Apr 2019
SRR6576653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323494
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6110.18887521870575652No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3780.11684915330732563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3570.11035753367914089No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3250.1004655418647641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGGT804.632966E-946.986947
CCGCTAA450.001014260641.7661674
ATAGACC500.001702542837.589553
GTATCAA8950.037.2976151
CATGGGT4150.036.230894
TACATGG18400.034.495442
GTACATG18700.034.4448851
TCTAGCG557.7063305E-834.17231828-29
ACATGGG18750.033.0788043
TCTTGCG1201.6411468E-731.3440062
CGCTAAA600.00416226131.3246235
AGCGCGA300.004165311331.32462310-11
CCCGCTA600.00416226131.3246233
GATAGTG600.00416226131.3246237
AACGCAG11350.030.2206736
GTCTAAA953.735337E-529.6943231
GTATATA1452.638808E-829.1823521
GTATAAG650.006137495428.9329281
CAACGCA11700.028.9150375
ATCAACG11600.028.7592473
Produced by FastQC (version 0.11.2)