FastQCFastQC Report
Sun 14 Apr 2019
SRR6576652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353305
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6590.18652439110683403No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3770.10670666987447107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.10387625422793338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG20450.038.386162
GTACATG20350.038.34381
CGTAGGT250.001701734537.59932328-29
ACATGGG20850.037.1935733
GTATCAA10100.036.301511
TACACTC801.1565255E-535.2493635
CTACACT1703.6379788E-1233.175874
AACGCAG11100.033.026436
TCGACCA300.004160274831.33276650-51
ACGACAG600.00415748531.3327665
ATCAACG11850.030.936153
TCAACGC12150.030.5591184
CATGGGA10800.030.0272374
CAACGCA12250.029.9259915
CATGGGG8400.029.6542264
CATGGGT4300.028.418094
TATATGT1503.6832716E-828.1994934
GGGTACC2700.027.8513497
ATGGGAG4750.027.7047625
ATGGGTA2950.027.0842555
Produced by FastQC (version 0.11.2)