FastQCFastQC Report
Sun 14 Apr 2019
SRR6576651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328843
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8130.24723044127440752No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4750.14444582977287032No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4040.12285497942787288No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3960.120422207558014No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3830.11646895326949336No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3770.1146443743670992No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3630.11038702359484617No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3510.10673786579005788No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3460.10521738337139608No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3390.10308870798526958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG20100.034.364073
ACTACTC550.002717713634.1728023
TACATGG21050.033.71122
GTACATG21400.033.379451
AGTAGTA851.7523289E-533.1727642
GTATCAA10550.032.072251
CTGCGCG600.0041620731.3250739
GGTACCT3400.030.4037448
CATGGGT5150.030.1085644
ATGGGTA3850.029.2909745
GTGTATA1205.83162E-627.4136091
AACGCAG11550.027.256886
GGGTACC3900.026.5058297
GATCAGG909.509376E-426.1081941
ATCAACG12250.026.0829163
TGGGTAC4150.026.0413236
TCAACGC12350.025.871724
CATGGGA9850.025.7597034
GTGCATA1101.0169429E-425.6335011
CAACGCA12300.025.5948755
Produced by FastQC (version 0.11.2)