FastQCFastQC Report
Sun 14 Apr 2019
SRR6576650_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576650_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365555
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8880.24291830230745035No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5360.14662636265404658No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4970.13595765343108424No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4800.13130719043645964No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4420.12091203786023992No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4310.11790291474607104No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4080.11161111187099068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.10723420552310871No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3910.10696064887636607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG11400.035.038814
ACATGGG25250.033.5001223
TACATGG26000.032.895262
GTACATG25750.032.756751
GTATCAA13300.031.887161
ATTAGCC600.004160236631.3288213
ATGGGGC3450.029.9666965
GCCTATA804.7025533E-429.4513361
CACTAGC650.006071266328.9982361
CGTTGTG350.00882837626.86062470-71
GCACGCG350.00882837626.86062470-71
ACGCGCG350.00882837626.86062472-73
AGATCCG700.00883448826.8532755
CCTAGAG700.00883448826.8532752
TCTATAC700.00883448826.8532753
CATGGGT5400.026.1073514
ATAAGGG909.513221E-426.107353
AACGCAG16150.025.8971986
CCCTTAA1109.986968E-525.7029841
TTAATAC1101.01747166E-425.6326713
Produced by FastQC (version 0.11.2)