FastQCFastQC Report
Sun 14 Apr 2019
SRR6576649_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576649_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359891
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7720.21450939312180634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.1330958540224679No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4170.11586841571475809No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3780.10503180129539222No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3750.1041982155708256No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3730.10364249175444787No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3710.10308676793807015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGT156.9030316E-493.983894
GTATAAG555.216543E-542.809171
GTACATG26950.034.5967981
ACATGGG26300.034.1272283
GTATCAA13550.034.0577741
TACATGG27300.033.7424932
ATGGGTA3300.031.3279635
GTCCTAC2350.030.05751
GTAGGAC2700.029.5875193
GTCCTAG1602.0809239E-929.4313031
CATGGGG9900.028.954634
AACGCAG15950.028.8727936
ATCAACG16300.027.9645293
CATGGGT5400.027.8470764
ATGGGAG5950.027.6423195
TCAACGC16500.027.6255664
TGGGTAC3250.027.4722126
CAACGCA17050.027.010035
ATTACAC1057.4163094E-526.8525353
TCCTAGA1956.020855E-1026.5119572
Produced by FastQC (version 0.11.2)