FastQCFastQC Report
Sun 14 Apr 2019
SRR6576649_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576649_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359891
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8390.23312614097046053No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4520.12559358250136846No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4500.12503785868499073No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4210.11697986334751355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.11225621090830279No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4000.11114476327554733No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3770.10475393938720334No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3710.10308676793807015No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3700.10280890602988127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12150.034.810491
ATAATAC1256.2573235E-933.8310973
GTCCTAC2550.033.1723481
TACATGG25200.032.2618682
ACATGGG25150.031.9478533
GTACATG25300.031.7628561
GTATATA1901.4551915E-1129.6805251
CATGGGG8800.027.7654194
CATGGGT5250.027.745084
CTACGAG2750.027.3420694
AACGCAG15250.026.806066
ATGGGTA3000.026.6263285
ATCAACG15900.026.3012543
TCAACGC15900.026.3012544
GTATTAT909.511825E-426.1078681
CAACGCA16300.025.6558255
CATGGGA14900.025.5436134
ATGGGAG5450.025.0025945
ACGCAGA16950.024.1175467
TCCTACA3350.023.8477862
Produced by FastQC (version 0.11.2)