FastQCFastQC Report
Sun 14 Apr 2019
SRR6576648_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576648_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399417
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7440.1862714906976919No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.11867296584772305No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4590.114917492244947No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4090.10239924690236019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG26750.037.0714652
GTACATG26700.037.0344121
ACATGGG27900.035.3749773
GTATCAA14000.034.6422841
GTTATAC1001.4085363E-632.8987273
GGGTACC3550.031.773427
CATGGGG8300.031.1433754
GGTACCT3700.030.4853128
ATCAACG16100.029.7752463
AACGCAG15900.029.5586076
ATGGGAG5950.029.2257615
TCAACGC16100.029.1914184
CATGGGA17250.028.6075924
CAACGCA16750.028.3392035
CATGGGT5650.028.2820954
CCTACAC1503.686546E-828.198913
TCCTATA1503.686546E-828.198912
TTATACC1205.832426E-627.4156074
ATGGGAT3550.026.477855
ATGGGTA3750.026.3189835
Produced by FastQC (version 0.11.2)