FastQCFastQC Report
Sun 14 Apr 2019
SRR6576647_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576647_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346200
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9940.28711727325245523No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6390.18457538994800693No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5940.17157712305025996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.16984402079722705No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5340.15424610051993068No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5060.146158290005777No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4650.134315424610052No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4490.1296938186019642No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4240.12247255921432698No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4030.11640670132871173No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3990.11525129982668979No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3990.11525129982668979No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3870.1117850953206239No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3750.10831889081455806No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3560.1028307336799538No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3560.1028307336799538No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG3470.10023108030040438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAT250.005235886756.392035
ATATACT708.594907E-846.993364
GTATCAA10450.043.302261
GTATAGT801.13358565E-535.3522381
AACGCAG12900.034.607516
CAACGCA12950.034.1110085
ATCAACG12900.033.514643
TCAACGC13050.033.1294174
TATATGC600.004156940631.333432
GTACTAG804.6937654E-429.4601971
ACTAGGC650.00615255828.918993
TACTCTC650.00615255828.918997
ACGCAGA15550.028.7097687
CGCAGAG16050.027.8153828
TATCAAC16350.027.5964152
ATGGGTA2800.026.8533485
TATATAC1406.354567E-726.8533483
TATACTC700.00883373626.8533485
CTAGGGT700.00883373626.8533484
GTACATG18300.025.4999751
Produced by FastQC (version 0.11.2)