FastQCFastQC Report
Sun 14 Apr 2019
SRR6576647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346200
Sequences flagged as poor quality0
Sequence length100
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11690.33766608896591566No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6060.17504332755632582No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5890.17013287117273254No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5220.15077989601386482No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5180.14962449451184287No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4620.13344887348353554No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4560.1317157712305026No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4430.12796071634893125No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4420.12767186597342578No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4300.1242056614673599No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4300.1242056614673599No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4210.12160600808781051No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4050.1169844020797227No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4020.11611785095320624No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4000.11554015020219525No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3920.11322934719815135No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3700.10687463893703061No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG3650.10543038705950318No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3640.10514153668399767No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA3610.10427498555748123No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3570.10311958405545926No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3480.10051993067590988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8000.047.5847931
CTATACT405.6768226E-446.990544
CCGAACT450.001013256141.7754021
CACTATC704.632253E-640.2776077
GTCCTAC1003.317109E-837.5978621
CAACGCA10450.035.973625
AACGCAG10600.035.9078676
ATCAACG10550.035.6326373
TCAACGC10650.035.298064
GCAAACG801.1567654E-535.2479931
TACATGG17200.031.9691142
GTACATG17250.031.8764481
CTAGTCT902.5922947E-531.3270284
CGCAAAA753.274715E-431.3270266
TGGGTAC4450.030.6230496
GGGTACC4300.030.5984947
ACGCAGA12450.030.1947257
ACATGGG18000.030.0217363
ATGGGTA4250.029.8528145
GGTATCA3650.029.6147561
Produced by FastQC (version 0.11.2)