FastQCFastQC Report
Sun 14 Apr 2019
SRR6576646_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576646_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224436
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4730.2107505034842895No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3280.14614411235274197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.14257962180755315No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2870.12787609830864924No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2530.11272701349159672No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2470.11005364558270508No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2450.10916252294640788No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2430.10827140031011069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTAC500.001681781837.6785161
ACGTTTG250.001702130737.59456686-87
GTATCAA7450.037.2992021
GTACATG15300.037.2475551
TACATGG15000.036.9679872
ACATGGG15000.036.0281263
ATGGGAG3250.033.256735
AACGCAG8800.031.5068056
CATGGGA8300.030.5738934
CAACGCA9400.028.9958085
ATCAACG9250.028.9579733
TCAACGC9550.028.04834
GTCTTAG856.716431E-427.7047921
CATGGGG5900.027.080834
TCTACAC1057.402726E-526.8532623
ATGGGAT2156.548362E-1126.2287655
ACGCAGA10600.026.1565957
CATGGGC2409.094947E-1225.4546534
TATCAAC10950.025.320542
CGCAGAG11000.025.2223058
Produced by FastQC (version 0.11.2)