FastQCFastQC Report
Sun 14 Apr 2019
SRR6576645_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576645_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239499
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4380.18288176568586925No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3140.13110701923598847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.12400886851302094No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2890.12066856229044798No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2750.1148230264009453No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2470.10313195462193997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7650.043.7837371
GTACATG14950.040.7065621
TACATGG15400.039.673742
ACATGGG15800.038.6693423
AACGCAG9600.034.2694636
CATGGGT4150.033.9745454
GTGTAGG1256.000846E-933.9663161
ATCAACG10000.033.3686643
CATGGGG6100.032.3593644
CAACGCA10200.032.2536135
TCAACGC10300.031.9404724
TAATACC600.004155279631.3320794
CTTAGGC600.004155279631.3320793
TATCAAC11300.030.777532
CATGGGA7850.029.3364054
ACGCAGA11400.028.4462327
ATATAGG1005.307643E-528.1988743
ATGGGAG2500.028.1988725
CGCAGAG11250.027.9899948
TGTAGGA1205.8197056E-627.415572
Produced by FastQC (version 0.11.2)