FastQCFastQC Report
Sun 14 Apr 2019
SRR6576644_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576644_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521748
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13330.2554873233821692No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9440.1809302575189555No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8140.1560140144284214No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7780.14911413172642732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.13991428812376855No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6690.12822282021205642No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6650.1274561665785015No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6480.12419788863589319No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6470.12400622522750447No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6330.12132293751006232No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6290.12055628387650744No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5970.11442305480806826No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5920.11346473776612463No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5740.11001479641512761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12850.048.7280961
TAACACG405.6776183E-446.9936754
GTACATG30350.037.849671
AACGCAG16550.037.197416
ACATGGG30650.037.1043053
ATGGGTA4750.036.60565
TACATGG31250.036.244992
ATCAACG16950.036.0423473
TCAACGC17000.035.936344
CAACGCA17150.035.896045
CATGGGT7900.034.5016824
TACTGCG702.1851E-433.566917
ATGGGAG6900.032.6912545
ACGCAGA19450.031.6512667
TATAGTC600.00416165931.3291175
TATCAAC20450.030.5660512
TAATACC1401.8892024E-830.2102184
CATGGGA13550.029.8262424
GACTTAT650.006100603428.9719411
CGCAGAG21450.028.9358228
Produced by FastQC (version 0.11.2)