FastQCFastQC Report
Sun 14 Apr 2019
SRR6576644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521748
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15140.2901784003005282No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9690.1857218427286736No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9110.17460536504212762No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8070.15467237056970032No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6940.1330144054217745No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6660.12764782998689023No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6530.1251562056778368No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6510.12477287886105935No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6200.11883131320100891No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6030.1155730352584006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.11116477686545995No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5780.1107814500486825No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5770.11058978664029379No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5490.1052232112054095No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC5490.1052232112054095No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG5380.10311491371313354No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5340.10234826007957865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14350.043.2382551
GTACATG31700.035.4392741
ATCAACG18050.034.622153
AACGCAG18900.034.3081176
GTACTAG550.002714174334.185621
ACATGGG32850.033.8994453
TACATGG33450.033.7257232
CAACGCA19150.033.6148645
TCAACGC18900.033.313684
ATGGGTA4950.030.3755655
ACGCAGA21350.030.151047
CATGGGT8650.029.8762574
GTATAGG1602.1263986E-929.3782651
CATGGGA13550.028.7818324
CGCAGAG22550.028.7549218
CATGGGG14850.027.844274
TATCAAC23200.026.9469622
AGATCGG700.00884341426.849835
GGTACCT5150.026.4588178
ATGGGAG6900.025.8770125
Produced by FastQC (version 0.11.2)