FastQCFastQC Report
Sun 14 Apr 2019
SRR6576641_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576641_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442927
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8130.1835516913622335No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5400.12191625256532115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.11491735658471938No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4820.10882154395645333No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4740.1070153772517819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13600.040.8646661
ATGGGTA3950.034.5042955
GTACATG26900.033.2664261
ATCAACG17000.032.8980643
CATGGGT6300.032.8234674
AACGCAG16850.032.633096
TACATGG27400.032.0747532
CAACGCA17800.031.4194985
TCAACGC17850.031.06824
ACATGGG28300.030.7225743
GGTACCT4200.030.2125078
GTAGTAT650.00615190728.9213753
GGGTACC4500.028.1983417
TATCAAC20300.028.013132
GTACCTG4700.027.9983529
TATACAG3100.027.2887155
CGCAGAG20300.026.8555628
ACGCAGA20450.026.6585797
TGGGTAC5150.026.464466
GTATAAT2202.3828761E-923.549131
Produced by FastQC (version 0.11.2)