FastQCFastQC Report
Sun 14 Apr 2019
SRR6576638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269879
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6030.22343346462674013No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3500.12968774895416094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.1200538018889947No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3190.11820111976107812No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3150.11671897405874485No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3010.1115314641005784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8050.036.79851
GTACATG15950.035.6705741
TACATGG16500.034.4815562
ACATGGG17250.033.781313
TGCCCGC600.00414661331.3468721
CATGGGT3600.031.3294414
ATCAACG9450.030.8321483
TCAACGC10050.029.4590244
CTACACG804.7747733E-429.3713534
CATGGGA8500.029.3022424
AACGCAG10050.028.9860526
TACACGG650.0061552428.9141275
ATGGGTA2450.028.7666055
CAACGCA10350.028.1458765
ATGGGGG2700.027.8432355
GGTACCT2305.456968E-1226.5569978
ATGGGAG3900.026.5046165
CGCAGAG11600.025.5179628
ACGCAGA11500.025.3312877
GTGTAGG1301.0801836E-525.3186261
Produced by FastQC (version 0.11.2)