FastQCFastQC Report
Sun 14 Apr 2019
SRR6576637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304939
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6400.20987804118200692No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3670.12035193924030708No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3580.11740052928618509No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3550.11641672596814445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.11215357825663493No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3400.11149770937794116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7500.040.1307531
GTATTAA853.924397E-738.7291261
GTACATG19000.036.1374741
TACATGG18800.036.0216182
ACATGGG19150.034.6038133
CATGGGA10600.033.696234
ATACCCC702.181969E-433.5695534
ATCAACG9400.030.998273
CAACGCA9550.030.5113875
TCAACGC9700.030.0395584
AACGCAG9550.029.527156
GCTCTAT804.75599E-429.392641
ATGGGAG5600.027.6948835
TATATCC700.0088284326.8556444
TATCAAC11050.026.8124732
CATGGGG8100.026.6898674
GCATTAT909.468341E-426.1267911
GTATAGA950.001297838824.7516961
TAGGACA1151.3744908E-424.520374
ACGCAGA11950.023.597017
Produced by FastQC (version 0.11.2)