FastQCFastQC Report
Sun 14 Apr 2019
SRR6576635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363111
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8750.24097314595261501No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4910.13522035961455314No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4570.12585683165753722No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4220.11621790581943263No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.114014722770723No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3950.10878216303003764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTA250.001703930437.5895970-71
GTATCAA11300.035.760011
ACATGGG21950.035.534583
GTACATG21650.035.375881
TACATGG21800.035.3480072
ATGGGGA4400.034.1723565
CATGGGG9200.032.68664
AATAGAC600.004162905331.3246613
AACGCAG13250.029.7879776
ATCAACG13750.029.04653
GTGTTCG650.00615702328.915079
TCAACGC13750.028.7047774
CAACGCA13950.028.293245
TCTATAC1005.3236843E-528.1921923
TGCCCGC1406.3635343E-726.8497091
CCCGCAA700.00884013526.8497093
CCTAGAC909.520473E-426.1038823
ACGCAGA15450.025.5463227
CATGGGA10850.025.1174684
TATCAAC15950.025.0400892
Produced by FastQC (version 0.11.2)