FastQCFastQC Report
Sun 14 Apr 2019
SRR6576634_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576634_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409530
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7680.18753204893414402No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5770.14089321905599103No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4780.116719165873074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.11232388347618003No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4490.10963787756696701No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4310.105242595170073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.043.0402071
TACATGG23850.037.838592
GTACATG24300.036.8410031
ACATGGG24900.036.0542223
GGGTACC3300.034.1795547
AACGCAG13000.033.9865156
ATCAACG13050.033.1359523
CATGGGT5450.032.7724724
TCAACGC13500.032.0314184
CAACGCA13650.032.0237665
GGTACCT3500.030.8836698
ATGGGTA3950.029.7484975
ACGCAGA15300.028.8739037
CATGGGG11000.028.6288724
CATGGGC3650.028.3303494
TGGGTAC3850.028.079496
GTATTAC856.7096885E-427.7162971
TATCAAC16650.027.6652092
CGCAGAG16150.027.0632238
GTATAAT2104.7293724E-1126.9244021
Produced by FastQC (version 0.11.2)