FastQCFastQC Report
Sun 14 Apr 2019
SRR6576633_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576633_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404200
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6920.17120237506185057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.14101929737753588No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4620.11429985155863434No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4370.10811479465611083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11250.043.944751
TCTAACG555.2717485E-542.7344742
GTACATG25800.036.4989621
TACATGG25550.036.428842
ATGGGAG5900.035.8446275
ACATGGG26700.035.2032133
AACGCAG13900.035.162696
CTAACGC550.002715985334.179123
TCAACGC14450.033.8243184
ATCAACG14500.033.7076843
ATACCGT851.7529279E-533.173856
CAACGCA14800.033.024425
CATGGGA15850.032.022714
CATGGGG8650.030.4253434
ATGGGTA3450.029.9686475
ACGCAGA16300.029.6933697
TATCAAC17000.029.0343042
CGCAGAG17000.028.488328
CCTATAC856.811535E-427.6448753
GCAGAGT19300.024.606099
Produced by FastQC (version 0.11.2)