FastQCFastQC Report
Sun 14 Apr 2019
SRR6576633_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576633_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404200
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7720.19099455714992578No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4840.11974270163285503No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4650.11504205838693715No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4320.10687778327560613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12450.043.7933581
TCAACGC15150.035.3593564
CAACGCA15250.035.127495
ATCAACG15300.035.0126953
AACGCAG15350.034.8986476
GTACATG26000.034.8902051
TACATGG26550.034.1716652
ACATGGG26600.033.9181143
TATCAAC17250.031.0662692
CATGGGA16350.029.8901214
CGCAGAG18150.029.2559328
ACGCAGA18300.029.0161297
ATGGGAG6500.028.9173685
CATGGGT6200.028.8007664
GTATAGC1555.0638846E-827.2916891
GACGTAA350.00884834526.84851854-55
ATGGGAC3600.026.1059575
TCGCTAC459.533077E-426.10272812-13
CATGGGG7600.025.9685574
CTAGTAC1101.01810176E-425.6313043
Produced by FastQC (version 0.11.2)