FastQCFastQC Report
Sun 14 Apr 2019
SRR6576632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448545
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8770.19552107369383226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.1074585604565874No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4720.10522912974172045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13250.047.536871
AACGCAG16550.038.0539746
ATCAACG17000.036.493733
TCAACGC17050.036.386714
CAACGCA17300.035.5892145
GTACATG31050.035.1210941
TACATGG31600.034.6585582
ACGCAGA19050.032.81337
ACATGGG33000.032.1875343
TATCAAC19600.032.135892
CGCAGAG19500.032.056078
CATGGGA17300.029.8840754
TACACCG804.776588E-429.3746875
CATGGGT7050.029.3330214
ATGGGAG6900.028.608395
GCAGAGT22600.027.866969
GGTACCT3800.027.2102368
ATGGGTA4050.026.6910725
GTATTAC950.001302580524.7393341
AGAGTAC20700.023.84032410-11
Produced by FastQC (version 0.11.2)