FastQCFastQC Report
Sun 14 Apr 2019
SRR6576631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296769
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9160.30865757542061334No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5610.1890359168241966No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4870.1641006978491689No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4770.16073107366335432No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3850.1297305311538604No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3780.12737179422379022No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3750.12636090696804586No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3500.11793684650350947No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3230.10883886120181016No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3190.10749101152748433No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3120.10513227459741416No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3120.10513227459741416No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3050.10277353766734396No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3050.10277353766734396No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3040.10243657524876251No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2990.10075176315585523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG708.596908E-846.9882057
TACCGTC450.001013990541.7672927
AGAACCG802.455181E-741.114685
GTACATG17900.039.90061
ACATGGG18300.039.0284583
TACATGG18800.037.9904672
ATAGAAC1551.8189894E-1236.3779643
GGTACCT3850.035.393718
CATGGGG7150.034.8304184
CATGGGT5000.033.831514
GTTATAG702.184331E-433.5630043
TGGGTAC4100.033.2355586
GGGTACC4100.033.2355587
ATGGGTA4250.033.1681445
GTATCAA9250.031.4947971
ATGGGCG753.2745497E-431.3254725
GTCTATA600.00416116331.3254721
CCGAACT753.2745497E-431.3254721
GAACCGC1351.3233148E-831.3254686
AACGCAG9500.030.6659856
Produced by FastQC (version 0.11.2)