FastQCFastQC Report
Sun 14 Apr 2019
SRR6576630_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576630_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323134
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8390.2596446056434792No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5440.1683512103337934No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4880.15102093868178526No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4720.14606943249549723No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4340.13430960530306313No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3810.11790774106098399No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3650.11295623487469594No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3590.11109942005483793No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3580.11078995091819493No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3580.11078995091819493No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3360.10398162991204886No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3340.10336269163876287No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3240.10026800027233285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG17650.038.0781333
GTACATG18650.037.0501481
CTTAGAC651.4115016E-436.1527183
TACATGG19350.035.709832
CATGGGT4800.034.269764
CATGGGG7750.033.3537944
GTATCAA8750.030.6209241
ATGGGTG1859.094947E-1230.4855335
TGGGTAC2950.030.270246
AAACGGA650.0061486528.9221746
CTAGTAC650.0061486528.9221743
TAGTACT650.0061486528.9221744
GACGCGC505.32111E-528.19911860-61
GGTACCT3200.027.9053768
CTAACCC700.00882814126.8563023
ATATAGG700.00882814126.8563023
TATAGGT700.00882814126.8563025
CATGGGA8050.026.2724694
GGGTAAG909.495977E-426.1143361
ATGGGTA3650.025.7526195
Produced by FastQC (version 0.11.2)