FastQCFastQC Report
Sun 14 Apr 2019
SRR6576629_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576629_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254507
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6200.24360823081486954No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4550.1787770080980091No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3480.13673494245737838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.1300553619350352No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3230.12691202992452075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3080.12101828240480615No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3020.11866078339692032No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2930.11512453488509158No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2910.11433870188246296No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2710.10648037185617684No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2580.10137245733909087No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG550.002715668934.1751025
GTACATG20600.032.925541
TACATGG20750.032.8433722
CATGGGT4150.032.836924
ACATGGG19550.032.4488683
CATGGGG6900.032.0082054
ATGGGTA2800.031.8865935
GGCTTAT600.00411116531.4012091
TAGACTG600.004158950431.3271755
TACACGG600.004158950431.3271755
GTAGTGC600.004158950431.3271756
CATGGGC2950.030.2652424
CTACACG650.006151192428.9173954
GGTACCT2800.028.5469348
ATACTGA1005.314602E-528.194466
TGGGTAC2850.028.0295816
TAAGGTC350.008835711526.85450656-57
GTAGGAC909.508157E-426.1059823
ATGGGGA3550.025.1499865
AATACTG1351.4538649E-524.3655835
Produced by FastQC (version 0.11.2)