FastQCFastQC Report
Sun 14 Apr 2019
SRR6576629_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576629_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254507
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6190.24321531431355523No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5050.1984228331637244No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3560.13987827446789283No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3500.137520775460007No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3390.13319869394554962No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3220.12651911342320643No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3010.11826786689560602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.11119536987194852No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2780.10923078736537697No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2700.10608745535486254No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2640.10372995634697671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC250.005237953756.382083
GTCTATA500.00169235737.6324161
TTTACAC1204.1927706E-935.23883
GCCCTAA550.002701609634.211291
GTTGTAC550.002717283834.170963
CGTGTAC550.002717283834.170963
TTGCATA550.002717283834.170964
ATATAGG851.7532502E-533.165934
CATGGGG7350.031.9626334
TATAGGA902.5916781E-531.323385
CTATACT1551.5133992E-930.3129484
ACATGGG21100.030.2842143
GTACATG21800.029.9937251
TACATGG21750.029.84642
ATGGGGA3450.028.5996075
CTGTGTA1154.1898893E-628.5996079
TACAGTC856.8140554E-427.6382777
GTCCTAT1057.355982E-526.8802971
ATGGGAG3150.026.8486125
GTACCCC700.00883701626.8486124
Produced by FastQC (version 0.11.2)