FastQCFastQC Report
Sun 14 Apr 2019
SRR6576627_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576627_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122205
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2750.2250317090135428No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1920.15711304774763715No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1610.13174583691338326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.12519945992389836No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1470.1202896771817847No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1320.10801522032650056No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG555.233463E-542.7574271
ACTATAC450.00101096141.773053
TACATGG13650.040.969722
GTACATG14000.040.3141441
ATGGGAC2000.039.945485
ATGGGAG2950.039.8265
GTATCAA7800.039.7972951
TCCTAAA608.783715E-539.162232
ACATGGG14300.039.107463
CATGGGA9750.037.1137474
AACGCAG8350.036.5826876
ATGGGAT2900.035.6511425
GGGAGAT801.15110215E-535.246017
CATTATA702.1750864E-433.5676272
ATCAACG9200.033.2027663
CATGGGT1850.033.023294
TATCAAC9500.032.6489372
TCAACGC9400.032.4963234
CAACGCA9600.031.8193155
GTCTTAA600.00413227431.3554481
Produced by FastQC (version 0.11.2)