FastQCFastQC Report
Sun 14 Apr 2019
SRR6576627_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576627_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122205
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.22666830326091406No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1540.126017757047584No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1410.11537989443967105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1330.10883351745018617No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1320.10801522032650056No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC255.547054E-575.179381
CGTTGCT408.004221E-658.73389494
CCGCTAA250.00523055656.3845374
CGCTAAA250.00523055656.3845375
GGTATAT250.00523055656.3845371
TGCCCGC352.9310794E-453.6995541
ATGGGCA1101.7735147E-938.4445
ACGTCCC250.001689756637.64359744-45
GTATAAT757.4031286E-637.5896871
TACATGG13400.033.6624072
GTACATG13500.033.413061
GTATCAA6800.033.167371
CATGGGC1900.032.1490754
ACATGGG13800.032.005713
TTAGGCA600.00415214931.3247414
TAATAGA600.00415214931.3247414
GGGAGAT600.00415214931.3247417
TGGGAGA1201.635799E-731.3247416
ATGGGAT2600.028.9151465
GTTCTAG650.00614118628.9151461
Produced by FastQC (version 0.11.2)