FastQCFastQC Report
Sun 14 Apr 2019
SRR6576619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264912
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5420.2045962432807876No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3030.11437760463852147No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3030.11437760463852147No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2780.10494050854623423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.1022981216403938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG17400.038.626441
ACATGGG17300.038.306363
TACATGG17550.037.7606852
GTCACGA250.001701199137.60000278-79
ACCTTAT757.421957E-637.6000021
CTAGTAC702.1804837E-433.571433
CATGGGG7100.033.0985954
CATGGGA7950.032.515724
TCTACCG300.00415897631.33333466-67
CTACCGA300.00415897631.33333468-69
CTCGATA300.00415897631.33333464-65
CGATAAT300.00415897631.33333466-67
CATGGGT3200.030.8437524
TCTATAC1401.8793799E-830.2142873
GTATCAA8450.029.4792881
ATGGGCG650.0061457428.9230775
CCTTATC1005.308298E-528.22
ATGGGAG4200.026.8571435
CGCCAAT350.008831826.85714314-15
ATGGGAT2650.026.6037735
Produced by FastQC (version 0.11.2)