FastQCFastQC Report
Sun 14 Apr 2019
SRR6576616_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576616_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327246
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7760.23713047676671373No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4210.12864939525616814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.11825965787205955No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3550.10848108151054559No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3550.10848108151054559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA3000.043.8617445
CATGGGT4600.037.8001064
GGGTACC3100.036.3830157
TACATGG20400.035.706782
GTACATG20650.035.5346571
ACATGGG20300.035.188173
GTATCAA10300.035.1642271
TCGTTCC550.002705512834.204129
ATATACG2200.034.1779826
TTAACCC702.1830386E-433.567664
TACGCTA2250.033.444039
TATACGC2250.033.4184727
GGTACCT3300.032.778958
TGGGTAC3850.031.7366986
ATACGCT2400.031.3537778
CCTATAC753.2726134E-431.3298173
ATTAGTC600.004158955531.3298153
AACGCAG12200.029.2754046
ATCAACG12450.029.0650123
CATGGGG7600.027.8258254
Produced by FastQC (version 0.11.2)