FastQCFastQC Report
Sun 14 Apr 2019
SRR6576616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327246
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7280.22246261222444277No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4180.1277326537222762No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3910.11948197991724879No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3900.11917639940595148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11400.039.579531
TACATGG22100.035.729032
GTACATG22250.035.69941
ACATGGG21950.035.3200153
ATAGGTT550.00271805734.171875
TACCTAG550.00271805734.171877
AACGCAG13000.033.9747286
ATCAACG13100.033.35673
TCAACGC13350.033.0840034
CATGGGT4300.032.7811554
CAACGCA13600.032.475845
TATCAAC14900.030.2823242
ACGCAGA14700.029.726047
CGCAGAG15100.028.9385978
ATGGGAG5950.028.42875
TTACTAC1005.3226107E-528.1917933
CATGGGG7900.027.9538884
CATGGGA13900.027.380524
CTCTAGG909.502265E-426.1114941
TAGTACA909.5192395E-426.1035144
Produced by FastQC (version 0.11.2)