FastQCFastQC Report
Sun 14 Apr 2019
SRR6576615_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576615_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361191
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7170.19850992964940986No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4700.13012505848706085No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3980.11019100697414941No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.10769925053503548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10650.039.7988551
ACATGGG20200.037.691073
TACATGG20550.037.277832
GTACATG20850.036.8179171
AACGCAG12800.032.6779566
CATGGGG7650.032.5603644
CAACGCA12950.032.299455
TCAACGC13300.031.4494614
ATCAACG13250.031.2134423
ATGGGAT2750.030.7620055
GTCCTAT1303.2625394E-728.9817281
GAGCGCG650.006150251728.9215419
CATGGGA9700.028.5861154
ACGCAGA14750.028.3578197
CATGGGT5650.028.2816854
CGCAGAG14950.027.9784498
TAGTACT1354.6220157E-727.8503764
CATGGGC3550.027.8013444
ATGGGTA4150.027.1792835
TATCAAC15250.026.5035112
Produced by FastQC (version 0.11.2)