FastQCFastQC Report
Sun 14 Apr 2019
SRR6576612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375514
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10430.2777526270658351No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5830.15525386536853486No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5830.15525386536853486No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5760.15338975377748898No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5550.14779741900435137No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4900.1304878113732111No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4610.12276506335316394No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4150.11051518718343391No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4130.10998258387170652No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3990.10625436068961477No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3970.10572175737788739No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT3880.1033250424751141No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3860.10279243916338672No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3850.10252613750752301No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA3830.10199353419579563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA200.002161891270.49966
GTAGCGT352.935042E-453.713986
CCTAGTA902.5847017E-531.3415032
CTAGTAC1052.060564E-631.3331553
ACATGGG24050.030.4863113
ATGGGTA3550.030.450535
GTACATG24450.030.3801061
TACATGG24950.029.7712862
GTCCTAC1303.313944E-728.9306181
GTACGTG650.006141136428.9306181
TAGTACA650.00614913428.9229134
GTATCAA12650.028.6161561
CATGGGG9700.028.5874654
ATATACT1205.8297173E-627.416514
CATGGGT5900.027.0845934
TCAAACG700.00882882926.856993
CTAGACA1301.0853346E-525.3075494
AACGCAG14450.025.044846
ATGGGGA6200.024.2579275
GTCTAAC1000.001753821223.5061261
Produced by FastQC (version 0.11.2)