FastQCFastQC Report
Sun 14 Apr 2019
SRR6576611_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576611_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125189
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3500.2795772791539193No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2150.17174032862312183No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2130.1701427441708137No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2010.16055723745696507No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1980.15816086077850292No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1970.15736206855234885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1790.14298380848157585No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1720.13739226289849746No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1620.1294043406369569No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.1262091717323407No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1560.12461158728003258No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1500.11981883392310827No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1410.11262970388772177No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1290.10304419717387311No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1260.10064782049541093No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1260.10064782049541093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACTA250.005228428656.3911324
ACCCCCT250.005228428656.3911327
TATAGTA450.001011274241.771212
CATGGGT2400.039.160514
ACATGGG9300.037.8972663
TACATGG9200.037.79842
CTATGGG500.001690892637.6241539
ATGGGAG1251.4915713E-1037.5940865
GTACATG9550.036.9938661
GGGTACC1353.4015102E-1034.809347
TGGGTAC1405.020411E-1033.566156
ATGGGTA1703.6379788E-1233.1712535
CATGGGG3600.032.633764
CCATATG300.00415802231.32840776-77
GTATCAA5700.030.5772721
AACGCAG5750.030.2387226
ATGGGGC953.7299178E-529.6795435
ATGGGGG1602.0991138E-929.370385
GTCTACA650.006138235328.9185282
CAACGCA6400.027.1676035
Produced by FastQC (version 0.11.2)