FastQCFastQC Report
Sun 14 Apr 2019
SRR6576609_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576609_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176988
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4150.2344791737292924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.1491626550952607No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2490.14068750423757542No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2440.13786245395168034No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2330.13164734332271114No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2310.13051732320835313No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2210.12486722263656293No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1840.10396185052093927No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1790.10113680023504418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGCA1357.2759576E-1238.2899635
GTGTATC500.001690099637.6363221
GTACATG13100.035.5533941
ACATGGG12850.035.4727333
TACATGG13150.035.3781782
CATGGGG5650.034.9322784
GTATTAA550.002698017734.214841
GTGTAGG702.1652461E-433.603861
GTACAAG600.00413197731.3636021
TATACTC600.004154922431.3281525
ATGGGGA3100.030.3175685
CATGGGC2101.8189894E-1229.0904274
ATGGGAG2101.8189894E-1229.0904275
GTATCCC650.00614526228.9182953
CTTTTAT1005.263695E-528.2272421
CGTATCA350.00879855826.87548470-71
GCCGTAT350.00879855826.87548468-69
TATCCTT700.0088233126.8527035
CATGGGA6000.025.0625234
AACGCAG8450.025.0254466
Produced by FastQC (version 0.11.2)