FastQCFastQC Report
Sun 14 Apr 2019
SRR6576608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196985
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5080.2578876564205396No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2760.14011219128360028No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2710.13757392694875245No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2680.13605096834784375No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2260.11472954793512197No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.11371424220118283No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2060.10457649059573064No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC2050.10406883772876108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAG250.005229618456.3999985
GGGTTAG601.5983205E-647.07
TAATACT450.001011908241.7777754
CATGGGG5800.039.7068984
CCGTCGT608.79747E-539.1666689
GGTTAGC757.412269E-637.6000028
TAGTTAT500.001698605537.62
CATGGGT3650.037.3424644
ACATGGG13950.036.050183
GTACATG13950.035.3763471
TACATGG14500.034.358622
GGTACCT1651.8189894E-1234.1818168
ATAGCAC702.178461E-433.571433
ATGGGGA3800.033.3947375
CCGATGA300.004157693631.33333414-15
GTGTAAT600.004152692431.3333341
ATGGGTA2051.8189894E-1229.8048785
TTAATAC804.7666772E-429.3749983
TAATGCG404.7752456E-429.37499818-19
GGGTACC2101.8189894E-1229.0952387
Produced by FastQC (version 0.11.2)