FastQCFastQC Report
Sun 14 Apr 2019
SRR6576607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405536
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9010.22217509666219523No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6280.15485678213524817No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5260.1297048843999053No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4920.12132091848812436No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4570.11269036534364396No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4400.10849838238775349No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4400.10849838238775349No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.10751203345695573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11100.043.6185651
TACATGG24650.038.3297732
GTACATG24850.038.0212861
ACATGGG24800.037.88973
AACGCAG13900.034.4769066
TCAACGC14150.033.8677674
CAACGCA14300.033.841075
ATCAACG14350.033.3957443
CATGGGT7000.032.888264
GGCGTAA300.00416753731.32215768-69
ATGGGGG3750.030.069275
ACGCAGA16550.028.9564327
CATGGGG9900.028.9492634
ATGGGTA4300.028.4084645
CGCAGAG17350.028.1628598
GGGTACC4350.028.081937
CATGGGA11650.027.8269754
TATCAAC17800.026.936262
ACCGGTG700.00884477926.8475637
GGTACCT4450.026.3950738
Produced by FastQC (version 0.11.2)