FastQCFastQC Report
Sun 14 Apr 2019
SRR6576605_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576605_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106005
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3060.2886656289797651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.22074430451393803No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2260.2131974906844017No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2140.20187726994009714No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1780.16791660770718364No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1690.15942644214895524No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1640.15470968350549502No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1630.153766331776803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1590.1499929248620348No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1470.1386727041177303No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1450.1367860006603462No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1440.13584264893165418No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1360.12829583510211784No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1340.12640913164473375No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1320.12452242818734967No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG1310.12357907645865762No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC1290.12169237300127353No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1290.12169237300127353No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1190.11225885571435311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTGC352.9143193E-453.7552958
TCTTACA405.658333E-446.9915052
CATGGGT2250.043.858744
ATGTGCA450.001006001541.8096739
CTTGCAC500.001696571837.593213
ATGGGTA1152.730303E-936.7759635
GTACATG11900.036.3810541
TGGGAGT651.4063477E-436.1473166
TGGGTAC1055.0456947E-835.8030556
GGTACCT1204.100002E-935.2769138
ACATGGG12100.034.9523623
ATGGGAG1750.034.9079785
ATGGGGG1353.3833203E-1034.8085255
CATGGGG3250.034.7014244
TACATGG12300.034.3840332
CTATATA550.002708346134.1756444
TTAGGGT702.1740078E-433.5653654
ATGGGGA1554.0017767E-1133.3488165
TCAGAGG753.2591284E-431.3276733
GGGTACC1351.3054887E-831.3276717
Produced by FastQC (version 0.11.2)