FastQCFastQC Report
Sun 14 Apr 2019
SRR6576603_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576603_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences844920
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13920.16474932537991763No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT10100.11953794442077356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.10557212517161388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15850.046.377571
GTACATG42350.037.607631
TACATGG42400.037.3678932
ACATGGG41400.037.1216933
ATCAACG20100.036.2423253
TCAACGC20150.035.9191474
GTATAAC1353.3651304E-1034.9042971
CAACGCA21050.034.829955
AACGCAG22400.032.7308246
GGTATCA7350.031.4138681
CATGGGG22150.029.0688064
TATCAAC25250.028.8605692
ACGCAGA25750.028.6551557
CATGGGA18350.027.404894
CGCAGAG27250.027.0778068
TAAGACG700.00883645826.856064
TACCGTA700.00883645826.856067
CATGGGT11550.026.4491524
TATAGGG2501.6370905E-1124.4476952
ATGGGTA6850.024.0136325
Produced by FastQC (version 0.11.2)