FastQCFastQC Report
Sun 14 Apr 2019
SRR6576600_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576600_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423385
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7810.18446567544905937No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5940.14029783766548176No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5140.12140250599336302No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5080.1199853561179541No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4630.1093567320523873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11600.044.3069271
GTACATG24650.037.6836051
TACATGG24600.037.6353262
ACATGGG25100.037.4473273
TCAACGC14850.034.812154
ATCAACG14800.034.6122133
CAACGCA15350.033.9843645
AACGCAG15700.033.825436
GCGTAGA600.004092694731.4348871
TATACCG600.004159600531.330935
CATGGGG9250.030.9922184
CATGGGT7000.030.8833454
ATGGGTA4050.030.1705275
CATGGGA13100.030.1350984
TATCAAC17250.029.6962742
ACGCAGA17950.029.3236567
CGCAGAG18300.028.5060128
GGTATCA6000.027.5055241
GTATATG1755.344191E-926.9441871
GCAGAGT19850.026.5168579
Produced by FastQC (version 0.11.2)