FastQCFastQC Report
Sun 14 Apr 2019
SRR6576598_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576598_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349235
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11750.33644966856128394No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7760.2221999513221756No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7200.20616490328861656No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6690.19156155597233956No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5990.1715177459303907No Hit
CTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATGGCAGC5920.16951336492619584No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5080.14546079287585723No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4770.13658424842870845No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4700.13457986742451358No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4610.13200280613340587No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4560.13057110541612382No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4520.12942574484229818No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA4520.12942574484229818No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4410.12627600326427765No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4400.12598966312082122No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4290.12283992154280068No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4210.1205492003951494No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4130.1182584792474981No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4030.11539507781293398No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG3700.10594585307887239No Hit
GTATACTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATG3690.10565951293541598No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3680.10537317279195957No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT3610.10336879178776469No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC3530.1010780706401134No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC3510.10050539035320057No Hit
GAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA3500.10021905020974416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.10021905020974416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA201.8339171E-594.022494
GTCTAGA405.592931E-447.1327551
GTATAAC405.592931E-447.1327551
ACTAGGC405.660575E-447.0179823
GTATCAA9700.044.2173271
TATAACG450.001011080241.793762
ATATACT450.001011793341.7877734
TAGCACT608.7990506E-539.1760374
GTACATG21650.038.3157731
TACATGG21950.037.7000662
TAAACCG250.00170196537.59822592-93
ACATGGG22500.037.1964453
TTACACT651.4094904E-436.1624954
GGGTACC5000.035.718317
CATGGGG11150.034.573294
TCAACGC12150.034.436224
TGGGTAC5200.034.3494536
ATCAACG12250.033.776183
ATATAGA702.1460424E-433.6662561
AACGCAG12450.033.6016166
Produced by FastQC (version 0.11.2)