SRR6576595_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR6576595_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	133837
Sequences flagged as poor quality	0
Sequence length	100
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	498	0.3720944133535569	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	285	0.21294559800354163	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	269	0.2009907574138691	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA	251	0.18754156175048753	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT	240	0.1793226088450877	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	232	0.1733451885502514	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	211	0.15765446027630625	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT	184	0.13748066678123388	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG	183	0.13673348924437936	No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG	182	0.13598631170752482	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC	181	0.1352391341706703	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG	179	0.13374477909696122	No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG	172	0.1285145363389795	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	168	0.12552582619156138	No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG	165	0.12328429358099777	No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT	164	0.12253711604414326	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT	164	0.12253711604414326	No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC	160	0.11954840589672511	No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC	152	0.11357098560188887	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC	148	0.11058227545447073	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	145	0.10834074284390713	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG	143	0.10684638777019807	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG	143	0.10684638777019807	No Hit
GAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA	138	0.10311050008592541	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	135	0.10086896747536181	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	15	6.893024E-4	93.98543	5
TATAACG	15	6.893024E-4	93.98543	3
GTACAAG	50	2.9648496E-5	47.08068	1
CATGGGC	175	0.0	37.59417	4
ATGGGCC	50	0.0016979515	37.59417	5
TACATGG	995	0.0	37.310795	2
GTACATG	1005	0.0	36.54023	1
CATGGGG	260	0.0	36.148243	4
GTATCAA	565	0.0	35.831314	1
ACATGGG	995	0.0	35.421642	3
TACTTCA	55	0.0027105366	34.17652	7
GTGTAGG	60	0.0041132737	31.38712	1
AAGACTG	75	3.2631826E-4	31.328474	5
AACGCAG	680	0.0	29.71598	6
ATGGGTA	180	2.3464963E-10	28.717768	5
CATGGGT	295	0.0	28.673521	4
CAACGCA	690	0.0	28.60426	5
GGTACCT	165	2.8958311E-9	28.501736	8
ATGGGAG	150	3.652167E-8	28.195627	5
ATCAACG	700	0.0	28.195627	3

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content