FastQCFastQC Report
Sun 14 Apr 2019
SRR6576594_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576594_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150681
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5330.35372741088790227No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2930.1944505279365016No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2780.18449572275203907No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2750.18250476171514657No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2380.1579495755934723No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2360.15662226823554395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.15064938512486645No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2260.14998573144590227No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2130.14135823361936808No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2040.13538535050869055No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1930.1280851600400847No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1920.1274215063611205No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1800.11945766221355048No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1690.11215747174494461No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1670.11083016438701629No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC1600.1061845886342671No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1600.1061845886342671No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1540.10220266656048207No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC1520.10087535920255374No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAC450.001011272341.7767453
GTATCAA4400.040.6844251
GTACATG9350.036.779731
TACATGG9500.036.6096232
CATGGGT3100.036.3861964
ACATGGG9650.036.040563
ATGGGGG1204.156391E-935.2491265
TACTATA550.002709883534.1809732
ATAACAC550.002709883534.1809733
GCGGTAT702.1468019E-433.6487771
TCCCGAC1151.1264456E-732.6948432
TGGGTAC1309.078576E-932.5376556
GGTACCT1457.3305273E-1032.412998
ATCAACG5650.031.6098383
ATGGGTA1807.2759576E-1231.332565
CATGGGC1351.3113095E-831.332564
ATGGGCA753.2626264E-431.332565
AACGCAG5550.031.332566
TTAGGCC600.00415012231.3325584
CGACCCG1201.6365266E-731.3325585
Produced by FastQC (version 0.11.2)