FastQCFastQC Report
Sun 14 Apr 2019
SRR6576592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426325
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14040.32932621826071656No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8070.18929220664985633No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7710.1808479446431713No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6870.16114466662757287No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6770.15879903829238257No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6420.15058933911921657No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA5870.13768838327566998No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5630.13205887527121327No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5630.13205887527121327No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5590.13112062393713717No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC5570.13065149827009911No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5520.12947868410250396No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5050.11845423092710959No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG5050.11845423092710959No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4870.11423209992376708No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4860.11399753709024806No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4740.1111827830880197No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4640.1088371547528294No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4550.10672608925115816No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC4450.10438046091596787No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT4440.10414589808244884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8800.045.4168851
CTCTATA450.00101113641.7954032
TACATGG22950.039.746612
GTACATG23000.039.6602061
GTATACC608.8172026E-539.164814
ACATGGG24450.037.0984463
AACGCAG11150.036.24946
GTCTAGA651.4084985E-436.16911
ATCAACG11000.035.8892063
TCAACGC11100.035.5658844
CAACGCA11150.035.406395
TGGGTAC4550.035.1192136
GTACATA959.401065E-734.646191
GGGTACC4750.033.640517
GGTACCT5050.031.6420638
CGCGATA300.00415896131.33552482-83
CATGGGT7700.029.9076734
ACGCAGA14550.027.7787517
CATGGGG13050.027.7304864
CGCAGAG15000.026.9453898
Produced by FastQC (version 0.11.2)