FastQCFastQC Report
Sun 14 Apr 2019
SRR6576589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281801
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5530.1962377706253704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.1185233551335872No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3060.10858726548166968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCG405.669386E-446.9999965
GTATCAA7600.042.0526351
TACATGG18650.037.8016052
TAACGCC500.001699848137.6000024
GTACATG18600.037.3978461
ATAACGC651.4107842E-436.1538473
ACATGGG19250.035.1584433
AACGCAG9950.032.12066
CATGGGT4250.032.0705874
ATCAACG9600.031.3333343
TCAACGC9750.030.8512824
CATGGGA10550.030.2938394
CAACGCA10150.030.0985225
GTACTTG1252.352499E-730.081
GTATTAT953.7423422E-529.6842121
GCCGTTC650.006146394628.9230778
ATGGGAG4950.027.5353555
TATCAAC11350.027.3303972
TAACTGA1555.0549716E-827.2903236
ACGCAGA11450.027.0917037
Produced by FastQC (version 0.11.2)