FastQCFastQC Report
Sun 14 Apr 2019
SRR6576588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295501
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5980.2023681814951557No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3860.13062561548015067No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3370.11404360729743723No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.10998270733432373No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3090.10456817405017241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA255.5607772E-575.177535
CCGCTAA352.9382663E-453.698244
GTCTTAT601.5931691E-647.0337031
AGTACCG405.6781934E-446.985965
GTACATG16950.044.6750871
TACATGG17600.042.4906772
ACATGGG17500.042.4216043
GTATCAA7100.042.396581
CCCGCTA500.001702479737.5887643
CATGGGA9000.034.4563674
ATCAACG9000.032.8901673
AACGCAG9150.031.8374796
TCAACGC9200.031.6644464
CATGGGG8200.030.9419694
CAACGCA9600.030.3450955
CTTATAG1102.9655184E-629.9001523
GTATAAT1602.1045707E-929.3960671
CATGGGT4750.028.6861614
ACGCAGA10200.028.5600937
ATGGGGA3900.027.7096655
Produced by FastQC (version 0.11.2)