FastQCFastQC Report
Sun 14 Apr 2019
SRR6576587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323205
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6340.19616033167803715No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3400.10519639238254358No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3340.10333998545814577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG405.673713E-446.9948167
GTATCAA7550.041.7104641
TACATGG19400.039.0068862
GTACATG19800.038.6936381
ACATGGG19900.038.4932443
CTAGTAC757.432647E-637.5958563
CATGGGA9900.032.7539634
TCAACGC9850.032.4431234
ATCAACG9750.032.2938773
ATGGGAG4200.031.3298785
TACCTAG600.004158841431.3298785
CGTTTGA300.00416189231.32987810-11
CAACGCA10300.031.0257055
AACGCAG10450.030.5803596
CATGGGG8900.030.0978034
GCCGCAA804.776453E-429.3717613
GGGTACC2950.028.6748057
GGTACCT3000.028.1968928
TATCAAC11200.028.1173212
GGTATCA4200.027.9774321
Produced by FastQC (version 0.11.2)